I-Polymerase chain reaction, eyaziwa kakhulu ngokuthi i-PCR, ingesinye sezindlela eziguqule kakhulu i-molecular biology ezake zathuthukiswa, enika abacwaningi amandla okukhulisa izingcezu ezincane ze-DNA zibe yizigidi zamakhophi phakathi namahora ambalwa nje. Kusukela yasungulwa ngeminyaka yawo-1980, i-PCR isibe ithuluzi elibalulekile ocwaningweni lwezakhi zofuzo, ekuxilongweni kwemitholampilo, kusayensi yezobunhloli, kanye nezinhlelo zokusebenza ze-biotechnology emhlabeni wonke. Ukusabela konke kuncike ku-enzyme ye-DNA polymerase ekwazi ukumelana namazinga okushisa aphezulu adingekayo ekuhlukanisweni kwe-DNA, futhi eyaziwa kakhulu kulezi yi-Taq polymerase, eyahlukaniswa ekuqaleni kusuka ku-thermophilic bacterium i-Thermus aquaticus. Le enzyme emangalisayo ihlala isebenza ngisho nasemazingeni okushisa adlula u-95°C, okuyivumela ukuthi isinde emjikelezweni wokushisa ophindaphindiwe oyisisekelo sayo yonke inqubo ye-PCR. Ngaphandle kwe-Taq polymerase, ukusebenza ngokuzenzakalelayo kwe-PCR bekungeke kwenzeke, njengoba ama-DNA polymerase ajwayelekile ayengahlukaniswa ngokushesha futhi angasebenzi ngaphansi kwezimo ezifanayo zokushisa. I-Ele Bam Bioscience itshale imali enkulu ekuthuthukiseni izinhlobo ezingcono ze-Taq polymerase kanye nezinto ezihambisana nazo ezinikeza imiphumela yokukhulisa enembile nethembekile ngaphansi kwezimo ezahlukahlukene zokuhlola. Ukuzinikela kwenkampani ekwenzeni ubunjiniyela be-enzyme kuqinisekisa ukuthi abacwaningi bangathembela ekuhlolweni kwabo kwe-PCR ukuthi kukhiqize idatha ethembekile ngaphandle kokukhungatheka ngenxa yokusabela okuhlulekile noma imikhiqizo yokukhulisa engaqondile.

Inqubo ye-PCR amplification yakhiwe ngezigaba ezintathu ezihlukene zokushisa eziphindaphindeka imijikelezo engama-25 kuye kwengama-40, isigaba ngasinye sidlala indima ebalulekile ekukhiqizeni kabusha ukulandelana kwe-DNA okuhlosiwe ngendlela ekhulayo. Ngesikhathi sokuhlukaniswa, ingxube yokusabela ifudunyezwa ifinyelele ku-94–98°C, okwenza i-DNA enemicu emibili ihlukane ibe imicu emibili eyodwa engasetshenziswa njengezithenjwa zokubopha ama-primer. Izixazululo zokuhlukanisa ezilungiselelwe kahle ze-Ele Bam Bioscience ziqukethe usawoti olinganiselwe kahle kanye nezinzisi ezivikela i-DNA template kanye ne-Taq polymerase enzyme emonakalweni obangelwa ukushisa ngenkathi iqinisekisa ukuhlukana okuphelele kwemicu umjikelezo ngamunye. Isinyathelo sokuhlanganisa silandela ngokushesha ukuhlukaniswa, lapho izinga lokushisa lehliselwa phakathi kuka-50°C no-65°C ukuze kuvunyelwe ama-oligonucleotide primers amafushane ukuthi abophe ngokuqondile ekulandelaneni kwawo okuhambisanayo ku-DNA template enemicu eyodwa. I-PCR master mixes ethuthukisiwe ye-Ele Bam ihlanganisa izithasiselo eziyimfihlo ezithuthukisa ukucaciswa kwe-primer futhi zinciphise amathuba okwakheka kwe-primer-dimer, okuyimbangela evamile yokwehluleka kwe-PCR kanye nokuncipha kokusebenza kahle kokukhulisa. Isigaba sokwandisa senzeka ku-72°C, izinga lokushisa elifanele ukusebenza kwe-Taq polymerase, lapho i-enzyme ihlanganisa khona umucu omusha we-DNA ohambisana ne-template ngokungeza ama-nucleotide ekugcineni kwe-3' kwe-primer ngayinye eboshiwe. I-Ele Bam ihlanganise izinhlobo zayo ze-Taq polymerase ukuze zibonise ukushesha kokwandisa kanye nokusebenza okuphezulu, okusho ukuthi i-enzyme ingafaka izinkulungwane zama-nucleotide ngomzuzwana ngaphandle kokwehla ku-DNA template ngaphambi kwesikhathi. Lokhu kunaka okucophelelayo esigabeni ngasinye sokushisa yikho okwenza izinto ze-PCR ze-Ele Bam zigqame emakethe egcwele, kunikeza abacwaningi ukuzethemba ukuthi ukukhulisa kwabo kuzosebenza okokuqala, njalo.

I-PCR yesikhathi sangempela, eyaziwa nangokuthi i-quantitative PCR noma i-qPCR, imele inqubekelaphambili ephawulekayo ngaphezu kwe-conventional endpoint PCR ngokuvumela abacwaningi ukuthi baqaphe inqubo yokwandisa ngesikhathi sangempela besebenzisa ama-fluorescent reporter molecules. Le ndlela inika amandla ukulinganisa okunembile kwe-DNA template yokuqala, okwenza kube yigugu ekuhlaziyweni kwe-gene expression, ekutholeni izifo, nasezifundweni zokuhluka kwenombolo yamakhophi emitholampilo nasemalabhorethri ocwaningo ngokufanayo. I-Ele Bam Bioscience inikeza uhla oluphelele lwama-reagents e-real-time PCR, okuhlanganisa ama-SYBR Green-based master mixes nama-probe-based kits ahambisana ne-TaqMan namanye ama-hydrolysis probe chemistries, wonke enziwe kahle ukuze athole ukuzwela okukhulu nokukhiqiza kabusha okuthembekile. I-reverse transcription PCR, evame ukufushaniswa ngokuthi i-RT-PCR, ihlanganisa ukuguqulwa kwe-RNA ibe yi-complementary DNA okulandelwa ukwandiswa kwe-PCR, ivumela abacwaningi ukuthi bafunde amazinga e-gene expression kusukela kumasampula e-RNA akhishwe kumaseli, izicubu, noma izibonelo zomtholampilo. Izixazululo ze-RT-PCR ze-Ele Bam zihlanganisa izinhlobo ezinyathelo olulodwa nezinyathelo ezimbili, zinika amalabhorethri ukuguquguquka ukukhetha indlela yokusebenza evumelana kangcono nezidingo zawo zokukhiqiza kanye nezintandokazi zomklamo wokuhlola. Ama-One-step RT-PCR kits ahlanganisa i-reverse transcriptase ne-Taq polymerase emgqeni owodwa wokusabela, anciphisa isikhathi sokusebenza futhi anciphise ingozi yokungcola, kuyilapho ama-two-step kits enikeza ukuguquguquka okukhulu ekwenzeni kahle ukusabela ngakunye kwe-enzymatic ngokuzimela. Ama-reagents e-real-time PCR ne-RT-PCR enkampani aqinisekiswe ngokuqinile emapulatifomu amaningi emishini, okuhlanganisa i-Bio-Rad CFX, i-Applied Biosystems QuantStudio, kanye nezinhlelo ze-Roche LightCycler, eqinisekisa ukuhlanganiswa okungenazinkinga emisebenzini ekhona yaselabhorethri ngaphandle kwezidingo ezengeziwe zokwenza kahle.

I-PCR amplification yaziwa kakhulu ngokuthi izwela ezinhlobonhlobo zezinto eziphazamisayo ezingavimbela ukusabela, zikhiqize amabhendi angacacile, noma zibangele ukwehluleka okuphelele kokusabela, okwenza ukwakhiwa kwe-reagent eqinile kube umehluko obalulekile phakathi kwabahlinzeki bezohwebo. Izithiyo ezivamile ezitholakala kumasampula ebhayoloji zihlanganisa i-heme evela kumasampula egazi, ama-humic acid avela emhlabathini nasezifundweni zemvelo, izinhlanganisela ze-phenolic ezivela ezicutshini zezitshalo, kanye nokugcwala okuphezulu kukasawoti noma izinto zokuhlanza ezivela ezindleleni zokukhipha. I-Ele Bam Bioscience ithuthukise ama-buffers akhethekile e-PCR kanye nezinhlanganisela ze-additive ezenzelwe ukumelana nalezi zinto eziphazamisayo, okuvumela ukukhuliswa okuphumelelayo ngisho nasezinhlotsheni eziyinselele zamasampula ezingahluleka kuzo izinto ezijwayelekile ze-PCR. Amandla e-multiplex PCR enkampani afanele ukukhulunywa ngokukhethekile, njengoba ukwakhiwa kwe-polymorphism ye-Ele Bam kanye nezinhlelo ze-buffer zivumela ukukhuliswa okuhambisanayo kwezakhi eziningi eziqondiwe emgodini owodwa wokusabela ngaphandle kokuncintisana okukhulu noma ukuchema phakathi kwama-amplicon. I-Multiplex PCR ibaluleke kakhulu ekuhloleni imitholampilo lapho ukuthola ama-pathogen amaningi noma izimpawu zofuzo kusampula elilodwa lesiguli kungonga isikhathi, kunciphise izindleko, futhi kulondoloze impahla yesampula elinganiselwe ukuze kuhlolwe okwengeziwe uma kudingeka. I-Ele Bam ilungise kahle ukugcwala kwe-magnesium ion, ukulinganisela kwe-dNTP, kanye nesilinganiso se-enzyme-to-buffer kuma-multiplex PCR master mixes ukuze kuqinisekiswe ukukhuliswa okulinganiselayo kuzo zonke izinto eziqondiwe, ngisho nalapho amasethi e-primer enezinga lokushisa elihlukile lokuncibilika noma ukusebenza kahle kokukhuliswa. Abacwaningi abasebenza nama-template e-GC-rich, ama-amplicon amade, noma amasampula e-DNA awohlokile babike imiphumela engcono kakhulu ngokuqhubekayo lapho besebenzisa izinto ezikhethekile ze-PCR ze-Ele Bam uma kuqhathaniswa nezinye izinto ezijwayelekile ezitholakala emakethe.

Ihubo lokuhlola lokuxilonga lomtholampilo seliyindawo ebaluleke kakhulu yokusetshenziswa kobuchwepheshe be-PCR, njengoba manje sekukhona izivivinyo ezitholakalayo zokuthola cishe wonke amagciwane abalulekile abantu, okuhlanganisa amagciwane, amabhaktheriya, isikhunta, nezimuncagazi, ngqo emithonjeni yeziguli. Izinto zokusebenza ze-PCR ze-Ele Bam Bioscience zisetshenziswe ezinhlelweni zokuxilonga ukuthola i-SARS-CoV-2, amagciwane omkhuhlane, i-hepatitis B ne-C, i-human papillomavirus, kanye nezinhlobonhlobo eziningi zokutheleleka ngamabhaktheriya, zibonisa ukuzwela nokuqina okungaguquki ezinhlotsheni ezahlukene zamagciwane. Ikhono lokuthola ukuphazamiseka kofuzo ngokuhlola okusekelwe ku-PCR liguqule izinhlelo zokuhlola ngaphambi kokubeletha, ukuhlola abathwali, nezinhlelo zokuhlola izingane ezisanda kuzalwa emhlabeni wonke, okuvumela ukungenelela kusenesikhathi kanye nezinqumo ezinolwazi zokuhlela umndeni ngokusekelwe emiphumeleni enembile yamangqamuzana. Izindlela ze-Taq polymerase ezinembile kakhulu ze-Ele Bam zilungele kakhulu izinhlelo zokusebenza ezidinga ulwazi olunembile lokulandelana, njengokuthola ukuguquka kofuzo emagcineni omdlavuza, ukuhlukaniswa kwezinhlobo zofuzo ze-single nucleotide polymorphisms, kanye nokulungiswa kwesifanekiso sokulandelana kwe-Sanger. Izinto zokusebenza zenkampani ziqinisekisiwe ezindaweni zokuhlola zokuxilonga ze-allele-specific PCR, i-methylation-specific PCR, kanye nezinhlelo zokusebenza ze-digital PCR, zinikeza abacwaningi bomtholampilo amathuluzi ahlukahlukene okuphenya izimo eziyinkimbinkimbi zofuzo. I-Ele Bam Bioscience isebenza eduze namalabhorethri omtholampilo ukuze iqinisekise ukuthi imikhiqizo yayo ye-PCR ihlangabezana namazinga eqiniso aqinile adingekayo ekusetshenzisweni kokuxilonga, okuhlanganisa ukuhlolwa kokungaguquki kwe-lot-to-lot, ukuqinisekiswa kokuzwela, kanye nezifundo zokuzinza ngaphansi kwezimo zangempela zokugcina nokuthutha.

Ukusebenza kahle kwelebhu esimweni sesayensi yamangqamuzana yesimanje kuncike kakhulu ekhwalithini nasekuthembekeni kwezinto ezisetshenziswayo kanye nama-reagents asetshenziswa emisebenzini yansuku zonke, lapho ama-PCR reagents engeminye yemikhiqizo esetshenziswa kakhulu kunayo yonke endaweni yocwaningo esebenzayo. I-Ele Bam Bioscience ikhiqiza i-Taq polymerase yayo namanye ama-enzyme e-PCR isebenzisa izinhlelo ezithuthukile zokukhiqiza amaprotheni kanye nezinqubo zokuhlanza ezinemingcele eminingi ezisusa ama-nuclease angcolile, ama-protease, neminye imisebenzi engafuneki yama-enzyme engaphazamisa imiphumela yokuhlola. Ama-buffer e-PCR enkampani alungiswa kusetshenziswa amanzi ahlanzekile kakhulu futhi ahlolwa ngokuqinile ukuze aqinisekise ukungabi bikho kwe-DNase, i-RNase, kanye nezithiyo ze-PCR, okuqinisekisa ukuthi yonke ibhetshi iletha ukusebenza okuphezulu okufanayo abacwaningi abakulindele ohlakeni lwe-Ele Bam. Ngokusebenzisa ama-master mixes e-PCR ahlanganisiwe e-Ele Bam, amalebhu anganciphisa kakhulu izinyathelo zokupipeta, anciphise ubungozi bokungcoliswa okuphambene, futhi asheshise isikhathi sokulungiselela izimpendulo ezijwayelekile ze-PCR, okuvumela ochwepheshe ukuthi bacubungule amasampula amaningi ngesikhathi esincane. Inkampani iphinde inikeze izinhlobo ze-hot-start Taq polymerase ezihlala zingasebenzi emazingeni okushisa asekamelweni, zivimbela ukwakheka kwama-primer-dimers kanye nemikhiqizo engaqondile yokwandisa ngesikhathi sokulungiselela ukusabela ngaphambi kokuqala kwe-thermal cycling. Lesi sici sibaluleke kakhulu emalebhu anomthamo ophezulu asebenza ngamakhulu ezimpendulo ze-PCR nsuku zonke, lapho ngisho nokuthuthukiswa okuncane ekucaciseni nasekuphindaphindeni kuhumushela ekongeni okukhulu ngesikhathi, ama-reagents, kanye nomzamo wokuxazulula izinkinga. Abacwaningi abanesithakazelo sokuhlola uhla oluphelele lomkhiqizo we-Ele Bam bangavakashela i-Imikhiqizo ikhasi ukuze uthole imininingwane eningiliziwe kanye nolwazi lokuyala.

Ukugcina izinqubo eziqinile zokulawula ikhwalithi elabhorethri ye-PCR kubalulekile ekukhiqizeni imiphumela ephindaphindekayo nethembekile, futhi ukusebenzisa ama-reagents asezingeni eliphezulu avela kumthengisi othembekile njenge-Ele Bam Bioscience kwakha isisekelo sanoma yiluphi uhlelo oluphumelelayo lokuqinisekisa ikhwalithi. Yonke i-experiment ye-PCR kufanele ihlanganise izilawuli ezifanele eziphozithivu nezingezinhle ukuze kuqinisekiswe ukuthi ama-reagents asebenza kahle nokuthi akukho ukungcola okwenzekile ngesikhathi sokulungiswa kwesampula noma ukumiswa kokuphendula. I-Ele Bam incoma ukuthi amalabhorethri asungule izindawo zokusebenza ze-Pre-PCR ne-Post-PCR ezinikezelwe ezinemishini namapipethi ahlukene ukuze kugwenywe ukungcola okuthwalwa yi-amplicon, okuyinto evame kakhulu ukubangela imiphumela engamanga emihle ekuhlolweni kwe-PCR yokuxilonga. Imikhiqizo ye-PCR yenkampani ikhiqizwa ngaphansi kwezinhlelo eziqinile zokuphatha ikhwalithi futhi ihlolwa ngokuphelele ngaphambi kokukhishwa, okuhlanganisa ukuhlolwa kwe-sensitivity, ukuqinisekiswa kwe-specificity, kanye nezifundo zokuzinza ezisheshayo ezilingisa izimo zokugcina zomhlaba wangempela. Ukuphepha elabhorethri nakho kufanele kube yinto ehamba phambili lapho usebenza nama-reagents e-PCR, njengoba ezinye izingxenye ezinjenge-nucleic acid dyes kanye ne-buffer additives ezithile zingaba yingozi empilweni uma zingaphathwa kahle. I-Ele Bam ihlinzeka ngamashidi edatha yokuphepha anemininingwane kanye nemihlahlandlela yokuphatha yayo yonke imikhiqizo yayo, futhi inkampani ikhuthaza abacwaningi ukuthi bahlale begqoka izinto zokuzivikela ezifanele, okuhlanganisa amagilavu namajazi elabhorethri, lapho bemisela ukuphendula kwe-PCR. Ukuze uthole izibuyekezo zakamuva zenkampani, ukwethulwa kwemikhiqizo, nezindaba zomkhakha, abacwaningi bangalandela i-Izindaba ikhasi ukuze uhlale unolwazi mayelana nokuqamba okusha okuqhubekayo kwe-Ele Bam Bioscience kubuchwepheshe be-PCR.

I-Ele Bam Bioscience isungule ukuthi ingumlingani othembekile emalabhorethri e-molecular biology ngokuletha njalo ama-PCR reagents ahlanganisa ukusebenza okukhethekile, ukuvumelana phakathi kwe-batch, kanye namanani ancintisanayo enza ucwaningo oluthuthukile lwe-molecular lufinyeleleke kumalabhorethri awo wonke usayizi. Ukuqonda okujulile kwenkampani nge-PCR biochemistry, kusukela ekwakhiweni kwama-thermostable polymerases kuya ekwakhiweni kwezinhlelo ze-buffer ezithuthukisiwe, kuvumela i-Ele Bam ukuthi inikeze imikhiqizo ebhekana nezinselelo zomhlaba wangempela ezibhekene nabacwaningi kanye nabahlaziyi bemitholampilo nsuku zonke. Ngokungafani nabahlinzeki abaningi abanikeza ama-reagents ajwayelekile angena ukuthuthukiswa okukhethekile kohlelo lokusebenza, i-Ele Bam ihlela izakhiwo zayo ukusekela izinhlobo eziningi zezinhlelo zokusebenza ze-PCR, okuhlanganisa i-PCR ejwayelekile, i-real-time PCR, i-RT-PCR, i-multiplex PCR, kanye ne-high-throughput screening workflows. Ukuzinikela kwenkampani ekusekeleni amakhasimende kubonakala emibhalweni yayo yezobuchwepheshe ephelele, ithimba elisabelayo lensebenzi yezobuchwepheshe, kanye nokuzimisela ukusebenza namalabhorethri ukuthuthukisa izinqubo zezinhlobo eziyinselele zamasampula noma imiklamo engajwayelekile yokuhlola. I-Ele Bam Bioscience nayo igcina ubudlelwano obuqinile namandla okusabalalisa emhlabeni wonke, iqinisekisa ukuthi amalabhorethri ezifundeni ezahlukene angathola imikhiqizo yayo ngokushesha nangokuthembekile ngaphandle kwezikhathi ezinde zokulinda. Ngokukhetha i-Ele Bam, amalabhorethri athola hhayi nje umhlinzeki we-reagents kodwa umlingani wesayensi ozinikele ekuthuthukiseni ucwaningo lwe-molecular kanye nokunemba kokuxilonga ngobuchwepheshe obuphakeme be-PCR. Ukuze ufunde kabanzi ngomsebenzi wenkampani kanye namagugu, vakashela i-Mayelana Nathi ikhasi.

I-PCR amplification, noma i-polymerase chain reaction, iyindlela yaselabhorethri eyenza izigidi zamakhophi engxenye ethile ye-DNA kusukela kusampula encane kakhulu yokuqala. Le nqubo isebenzisa ukushisa nokupholisa okuphindaphindiwe ukuze kushiswe futhi kupholise ingxube yokusabela, okuvumela ukuhlukaniswa kwe-DNA, ukunamathiselwa kwama-primer, nokwandiswa kwe-enzymatic emjikelezweni ngamunye, okuholela ekwandeni okukhulu kohlelo lwe-DNA oluhlosiwe. Ama-PCR kits akwa-Ele Bam Bioscience ahlinzeka ngazo zonke izinto ezilungiselelwe ezidingekayo ukuze kwenziwe le nqubo ngokuthembekile nangempumelelo kunoma yiliphi ilabhorethri ye-molecular biology.

I-Taq polymerase iyi-enzyme ye-DNA polymerase ekwazi ukumelana nokushisa, eyatholakala e-Thermus aquaticus, ehlala isebenza emazingeni aphezulu okushisa adingekayo ukuze i-DNA ihlukaniswe ngesikhathi se-PCR. Ikhono layo lokumelana nokushiswa okuphindaphindiwe kuze kufike ku-95°C ngaphandle kokulahlekelwa amandla okusebenza kwe-enzyme, liyenza ibe isisusa esihle kakhulu senqubo yokwandisa i-PCR, ivumela ukushisa okuzenzakalelayo ngaphandle kwesidingo sokwengeza i-enzyme entsha ngemva komjikelezo ngamunye. I-Ele Bam Bioscience inikeza izinhlobo eziningi ze-Taq polymerase, okuhlanganisa ne-hot-start kanye ne-high-fidelity variants, ukuze kuhlangatshezwane nezidingo ezahlukene ze-PCR kanye nezidingo zokuhlola.

I-PCR evamile ikhulisa i-DNA ngqo, kuyilapho i-RT-PCR (i-reverse transcription PCR) iqala ngokuguqula i-RNA ibe i-complementary DNA isebenzisa i-reverse transcriptase ngaphambi kokwenza i-PCR amplification. I-RT-PCR ibalulekile ekutadisheni amazinga okukhuluma kwezakhi zofuzo, ekutholeni amagciwane e-RNA, nasekuhlaziyeni idatha ye-transcriptomic, futhi isetshenziswa kabanzi emkhakheni wocwaningo kanye nasekuhloleni imitholampilo. I-Ele Bam Bioscience ihlinzeka ngamakhithi e-RT-PCR esinyathelo esisodwa kanye nezinhlangothi ezimbili enza lolu hlelo lube lula futhi inikeze imiphumela engaguquki nephindaphindekayo.

Yebo, i-Ele Bam Bioscience inikeza uhla olubanzi lwama-reagents e-PCR esikhathini sangempela enziwe ngokukhethekilele izinhlelo zokusebenza ze-quantitative PCR, okuhlanganisa ama-master mixes asuselwa ku-SYBR Green kanye nama-kits asuselwa ku-probe ahambisana namakhemikhali ahlukahlukene okuthola. Le mikhiqizo iqinisekisiwe kumapulatifomu amaningi e-real-time PCR futhi iletha ukuzwela, ukucacisa, nokuphindaphindeka okudingekayo ekuhlaziyweni okunembile kwe-gene expression kanye nokulinganisa kwe-pathogen.

I-Multiplex PCR iyindlela ekhulisa izintambo eziningi ze-DNA eziqondiwe ngesikhathi esisodwa emgodini owodwa wokusabela ngokufaka amapheya amaningi e-primer aqondiswe ezinhlosweni ezahlukene. Izinzuzo eziyinhloko zihlanganisa ukuncishiswa kwezindleko zamakhemikhali, ukusetshenziswa kwesampula okuncane, ukushesha ekutholeni imiphumela, kanye nekhono lokuthola ulwazi olwengeziwe kusampula elilinganiselwe lezokwelapha noma lobugebengu. I-Ele Bam's optimized multiplex PCR master mixes yakhelwe ukuvimbela ukuncintisana phakathi kwamasethi e-primer futhi iqinisekise ukukhuliswa okulinganiselayo kwazo zonke izinhloso.

I-Ele Bam Bioscience ikhiqiza ama-PCR reagents ayo ngaphansi kwezinhlelo eziqinile zokuphatha ikhwalithi futhi yenza ukuhlolwa okuphelele kokusebenza kuwo wonke amalotho okukhiqiza, okuhlanganisa ukuhlolwa kwe-sensitivity, ukuqinisekiswa kwe-specificity, kanye nezifundo zokuzinza okusheshisiwe. Ama-enzyme enkampani ahlanzwa kusetshenziswa izinqubo eziphambili ezinamanyathelo amaningi ezisusa ukungcola okungaholela ekuphazamisekeni, futhi wonke ama-buffers ahlolwa ukuthi awanawo i-DNase, i-RNase, kanye nama-PCR inhibitors ukuze kuqinisekiswe ukuvumelana phakathi kwamalotho.

Izinto ezivamile ezivimbela i-PCR zihlanganisa i-heme evela emasampuleni egazi, ama-humic acid avela kumasampuli emvelo, izinhlanganisela ze-phenolic evela ezicutshini zezitshalo, ukugxila okuphezulu kasawoti, kanye nezinsalela ze-detergents ezivela ezindleleni zokukhipha i-DNA. Ama-buffer akhethekile e-PCR kanye nezithako ezengeziwe ze-Ele Bam Bioscience aklanyelwe ukunqoba lezi zinto ezivimbelayo, okwenza ukukhulisa ngempumelelo ngisho nasemasampulweni ayinselele noma angahlanzekile okungabangela ukuthi izinto ezijwayelekile ze-PCR zehluleke.

Yebo, i-Ele Bam Bioscience ihlinzeka ngama-hot-start Taq polymerase formulations ahlala engasebenzi emazingeni okushisa asekamelweni futhi asebenze kuphela ngemuva kokuchayeka emazingeni okushisa aphezulu ngesikhathi sesinyathelo sokuqala sokuhlukanisa. Lesi sici sivimbela ukwakheka kwe-primer-dimer kanye nokukhuliswa okungaqondile ngesikhathi sokulungiselela ukusabela, okwenza kube yinto ekahle emalabhorethri anomthamo ophezulu kanye nezivivinyo ezidinga ukucaciswa okuphezulu nokuphindaphindeka.

Izilabhorethri kufanele zisungule izindawo zokusebenza ezihlukene ze-Pre-PCR ne-Post-PCR ezinemishini, amapiphethi, nezinto ezisetshenziswayo ezinikezelwe ukuze kugwenywe ukungcoliswa okubangelwa ukuthuthwa kwama-amplicon. Izindawo ze-Pre-PCR kufanele zisetshenziselwe ukuhlela ukusabela nokulungisa amasampula kuphela, kuyilapho izindawo ze-Post-PCR ziphethe ukushisa okujikelezayo nokuhlaziya okulandelayo, kusetshenziswa izinqubo eziqinile zokuhamba komsebenzi okuya ngakunye. I-Ele Bam Bioscience inikeza umhlahlandlela onemininingwane ngezinqubo ezinhle zaselabhorethri njengengxenye yezinsizakalo zayo zokusekela amakhasimende.

I-Ele Bam Bioscience PCR reagents zitholakala ngokusebenzisa abasabalalisi abagunyaziwe emhlabeni wonke, futhi imininingwane eningiliziwe yomkhiqizo ingatholakala ekhasini lenkampani elithi Ikhaya ikhasi kanye ne-Imikhiqizo ikhasi. Abacwaningi bangaphinde bahlole i-Uhlobo ikhasi ukuze uthole ulwazi olwengeziwe mayelana nokuzibophezela kwe-Ele Bam ekhwalithini nasekusungulweni kwezinto ezintsha kuma-reagents e-molecular biology.