I-Polymerase chain reaction, eyaziwa kakhulu nge-PCR, ingesinye sezinto eziguqule kakhulu umkhakha we-molecular biology kusukela yathuthukiswa ngeminyaka yawo-1980. Le ndlela eyisimangaliso ivumela ososayensi ukuthi bandise amanani amancane e-DNA abe yizigidi zamakhophi, okwenza ukuthi ukutholwa nokuhlaziywa kube nokwenzeka ngisho nasemanzini amancane kakhulu e-biological. Emashumini eminyaka, i-PCR isuke iyithuluzi eliyisisekelo laselabhorethri yaba yinsika engalungiseki yokuxilonga kwesimanje, inika amandla konke kusukela ekuhlonzeni amagciwane kuya ekuhloleni izakhi zofuzo. Ikhono lokuthola imiphumela enembile nephindaphindekayo ye-PCR libaluleke kakhulu ezindaweni zokwelapha, lapho ukungatholi umphumela oyiphutha kungabambezela ukwelashwa okusindisa ukuphila, futhi umphumela oyiphutha ongemuhle ungadala ukungenelela okungadingekile. Ekuxilongeni izifo ezithathelwanayo, isibonelo, ukuthembeka kwe-PCR assays kunomthelela ngqo ezinqumweni zokuphatha iziguli, izindlela zokulawula ukuqubuka kwezifo, kanye nezimpendulo zezempilo zomphakathi. Izinhlobo ezahlukene ze-PCR, ezihlanganisa i-conventional, nested, ne-multiplex, zithuthukisiwe ukuze kubhekwane nezinselele ezithile zokuxilonga, ngayinye inikeza izinzuzo ezihlukile ekuzweleni nasekukhiqizeni. Ukuqonda ukubaluleka okuyisisekelo kwe-PCR ekuxilongeni kusiza amalabhorethri ukuthi abone ukuthi ukutshala imali kumareagents namapulatifomu asezingeni eliphezulu akukhona nje ukuthola ukunethezeka kodwa kuyisidingo ukuze kutholakale imiphumela enokwethenjelwa.

Ukuziphendulela kobuchwepheshe be-PCR kusheshe kakhulu eminyakeni eyishumi edlule, kuqhutshwa yisidingo esiphuthumayo sezindlela zokuthola ezisheshayo, ezinomuzwa omuhle, futhi eziqondile ngokwengeziwe. I-PCR yesikhathi sangempela, eyaziwa nangokuthi i-quantitative PCR noma i-qPCR, isithathe indawo yezindlela zokuphela ngoba ivumela ukuqapha okuqhubekayo kokukhuliswa kanye nokulinganisa okunembile kwe-target nucleic acids. Lokhu kuthuthuka kube nenguquko ikakhulukazi ku-virology, lapho i-rt PCR assays isibe yindinganiso yegolide yokuthola ama-RNA virus afana ne-SARS-CoV-2, influenza, kanye ne-respiratory syncytial virus. I-enzyme ye-polymerase emgogodleni wayo yonke impendulo ye-PCR, evame kakhulu i-Taq polymerase, kumele ibonise ukumelana nokushisa okungavamile kanye nokusebenza kahle ukuze imelane nokushintshashintsha okushisayo okuphindaphindiwe ngenkathi igcina ukwethembeka okuphezulu. Izinto ezintsha ekwakhiweni kwe-enzyme ziholele ezinhlobonhlobo eziguquliwe ze-Taq polymerase ezinikeza ukumelana okuthuthukisiwe nama-inhibitors, amazinga okunweba asheshayo, kanye nokucacisa okuthuthukisiwe ngisho nasezimeneni eziyinkimbinkimbi zamasampula. Njengoba amalabhorethri emhlabeni wonke eqhubeka nokwandisa amandla awo okuhlola amangqamuzana, isidingo sezixazululo ze-PCR eziqinile eziletha ukusebenza okungaguquki kuzo zonke izinhlelo zokusebenza eziningi asikaze sibe sikhulu kangaka. I-Ele Bam Bioscience izibeke phambili kulo mkhakha, inikeza izinhlelo ze-PCR ezihlanganisiwe ezihlanganisa amakhemikhali athuthukile e-reagent kanye nokwakhiwa kwenkundla ehlakaniphile ukuze kuhlangatshezwane nezidingo eziqinile zokuxilonga kwesimanje.

I-Ele Bam Bioscience ithuthukise inqwaba yezinkundla ze-PCR zesikhathi sangempela kanye nezindlela zama-enzyme ezakhelwe ukuletha ukusebenza okuhle kakhulu ezindaweni zokwelapha nezocwaningo. Izinhlelo eziyinhloko zenkampani zakhiwe ngama-module okuthola ukukhanya angumnikazi anika amandla ukuqapha ukukhanya okuzwela kakhulu emihlangeni eminingi, okusekela izinhlobo eziningi zamakhemikhali okuhlola ezifana ne-TaqMan, i-SYBR Green, nama-molecular beacons. Iplatifomu ngayinye ye-Ele Bam ihlanganiswa ne-master mixes ekhethekile equkethe amazinga afanele e-buffer components, ama-dNTPs, kanye nohlobo olusha lwe-thermostable polymerase olumelana kakhulu nama-inhibitors avamile e-PCR atholakala egazini, emhlabathini, nasezicutshini zezitshalo. Ama-module okushisa aphindaphindayo anezinga lokushisa elisheshayo nokulawula izinga lokushisa okunembile, okunciphisa isikhathi sokusebenza sisonke sibe ngaphansi kwemizuzu engu-30 ekuhlolweni okuningi okujwayelekile ngaphandle kokuphazamisa ukusebenza kahle noma ukucacisa. Le nhlanganisela yokushesha kwe-cycling kinetics kanye ne-robust enzyme chemistry ivumela amalabhorethri ukuthi acubungule amasampula amaningi ngosuku ngenkathi egcina izinga eliphezulu ledatha elidingekayo ukuze kuthathwe izinqumo zokuxilonga eziqinisekile. Ukuzinikela kwe-Ele Bam ekusunguleni ubuchwepheshe kubonakala kuyo yonke ingxenye yezinhlelo zayo ze-PCR, kusukela kuma-algorithms esofthiwe ahlaziya amajika okukhulisa kuya ezintweni ezisetshenziswayo eziklanyelwe ukunciphisa ukuhwamuka nokudlulisa ukushisa okuhle. Ngokuhlanganisa lezi zinto ezinqubweni ezinomthombo omuhle, i-Ele Bam Bioscience inika amandla amalabhorethri ukuthi athole imiphumela ethembekile ngesikhathi esincane sokusebenza nokuphindaphindeka okukhulu phakathi kwabasebenzi abahlukene nezinsimbi.

Enye yezici ezihlukile zobuchwepheshe be-PCR ye-Ele Bam ukugcizelela kwayo amandla okwenza izinto eziningi ngesikhathi esisodwa, okuvumela ukutholwa kwezinhloso eziningi ngesikhathi esisodwa emgodini owodwa wokusabela. Lokhu kufinyelelwa ngamathuluzi okuklama ama-primer nama-probe acushwe ngokucophelela, kuhlanganiswe nezingxube zama-enzyme ezigcina umsebenzi ophezulu ebangeni elibanzi eliguquguqukayo ngisho nalapho ama-amplicon amaningi eqhudelana ngezingxenye zokusabela. Imishini ye-real-time PCR yenkampani ingahlukanisa imigudu emithathu yama-fluorescent ene-spectral overlap encane, ivumela abasebenzisi ukuthi baklame amaphaneli afaka izinhloso eziningi zezifo, izilawuli zangaphakathi, ngisho nezinkomba zofuzo lomnikazi konke ekuphenduleni okukodwa. La mandla okwenza izinto eziningi ngesikhathi esisodwa abaluleke kakhulu ekuxilongweni kwezifo ezithathelwanayo, lapho ukuhlukanisa phakathi kwamagciwane anezimpawu zomtholampilo ezifanayo — njenge-SARS-CoV-2, influenza A, influenza B, kanye ne-respiratory syncytial virus — kungenziwa esivivinyweni esisodwa kunokuba kudingeke izivivinyo ezihlukene. I-Ele Bam iphinde inikeze izingxube ezikhethekile zama-master mix ezenzelwe izinhlelo zokusebenza eziyinselele njengokukhulisa i-GC-rich template, ukuhlola isampula ngqo ngaphandle kokukhipha i-nucleic acid, kanye ne-one-step rt PCR yezinhloso ze-RNA. Inkampani inikeza imibhalo yobuchwepheshe ephelele kanye nokusekelwa ekuthuthukiseni izivivinyo ukusiza abasebenzisi ukuthi bathuthukise izinqubo zabo ukuze bathole ukuzwela nokuqondisa okuphezulu. Emalabhorethri abalulekile ukuguquguquka, ukwakheka kwe-Ele Bam okuvulekile kuvumela ukuhambisana nezinto zokusebenza zangaphandle kanye nezinto ezisetshenziswayo, kunikeza abasebenzisi inkululeko yokukhetha amathuluzi ahambisana kahle nomsebenzi wabo kanye nesabelomali sabo.

Inzuzo enkulu yamakhithi e-PCR akwa-Ele Bam isekuzweleni kwawo okuphezulu, okuvumela ukutholwa okuthembekile kwama-nucleic acid aqondiwe ngisho nasezingeni eliphansi kakhulu lekhophi. Lokhu kufinyelelwa ngokuhlanganisa ukubopha kwe-primer okunamandla okuhlangana, ukunwetshwa kwe-polymerase okusebenza kahle, kanye ne-chemistry ye-buffer elungiselelwe ukukhulisa izilinganiso zomsindo kuya kumsindo kusabelo ngasinye. Ngamazwi asebenzayo, lokhu kusho ukuthi amakhithi akwa-Ele Bam angathola ngokungaguquki amakhophi amahlanu kuya kwayishumi we-template eqondiwe ngokusabelo ngakunye, izinga lokusebenza elibalulekile ekuxilongweni kusenesikhathi kwezifo lapho umthwalo we-pathogen usuncane. Ukuzwela okunjalo kubaluleke kakhulu ezinhlelweni ezinjengokuhlolwa kwegazi ukuze kutholakale ama-pathogen atholakala egazini, lapho amazinga aphansi e-viral engalahleka kalula ngokuhlolwa okungazweli kangako, okungase kube yingozi ekuphepheni kwesiguli nasekuphepheni komnikelo wegazi. Ukuzwela okuthuthukisiwe nakho kuhumushela ekuzethembeni okukhulu kwezibalo ekuhloleni okulinganiselayo, okuvumela abacwaningi ukuthi babone izinguquko ezincane ekuvezweni kwezakhi zofuzo noma emthwalweni we-pathogen ngokuhamba kwesikhathi. U-Ele Bam ufinyelela leli zinga eliphezulu lokusebenza ngaphandle kokulahla ukucaciswa, njengoba ukwakheka kwabo kwe-enzyme kanye nezimo zokusabela kuklanyelwe ukunciphisa ukunwetshwa okungaqondile kanye nokwakheka kwe-primer-dimer. Emalabhorethri asebenza njalo namasampula ayinselele njengezicubu ezilungiswe nge-formalin ezifakwe ku-paraffin, izinhlobo zobufakazi, noma amasampula emvelo anezinto eziphazamisayo eziphezulu, ukuzwela okuqinile kwamakhithi akwa-Ele Bam kungenza umehluko phakathi kokuthola idatha esebenzisekayo nokwehluleka ukukhulisa ngokuphelele.

Ngaphezu kokuzwela okukhethekile, amakhithi e-Ele Bam PCR anikeza ukukhetha okuqinile okugwema ngempumelelo ukusabela okuhlangene nezinto eziphilayo ezihlobene kakhulu noma izifunda zofuzo ezingaqondiwe. Lokhu kukhetha kwakhiwa ngokuqinisekiswa okunzima komklamo we-primer ne-probe, kanye nokusetshenziswa kwama-nucleotide aguquliwe nama-annealing buffers akhuthaza ukwethembeka okuqinile kokubhangqa kwe-base ngesikhathi somjikelezo ngamunye wokushisa. Inkampani isebenzisa inqubo yokuqinisekisa enezigaba eziningi ehlola imiklamo yokuhlola ekhethiwe ngokumelene nezizinda ezinkulu zofuzo ezaziwayo ukuze kutholwe futhi kuqedwe izindawo ezingaba yinkinga zokusabela okuhlangene ngaphambi kokuba umkhiqizo ufinyelele emakethe. Ngenxa yalokho, amaphaneli ezifo ezithathelwanayo e-Ele Bam abonisa ukusabela okuhlangene okungasho lutho nezinto ezigulayo ezingaqondiwe ezihlanganyela ukufana kofuzo, kunciphisa ingozi yemiphumela engamanga engadida izinqumo zokwelapha. Ngaphezu kwalokho, isikhathi esisheshayo sokuphendula esinikezwa izinqubo ezisheshayo zomjikelezo ze-Ele Bam sivumela amalabhorethri anomthamo ophezulu ukuba aqedele amapuleti agcwele ama-96 noma ama-384 ngaphansi kwemizuzu engama-40, kuhlanganise nokuhlaziywa kwedatha. Lokhu kushesha kutholakala ngaphandle kwesidingo sezinsimbi ezikhethekile zokusheshisa umjikelezo — izinkundla ezijwayelekile ze-Ele Bam zakhiwe kusukela ekuqaleni ukuze kube nokulinganisela okusheshayo kokushisa nokudluliswa okusebenzayo kokushisa. Emalabhorethri acubungula amakhulu noma izinkulungwane zamasampula nsuku zonke, njengalawo asezikhungweni zokuxilonga eziphakathi noma ezikhungwen zomnikelo wegazi, lokhu konga isikhathi kuhlangana ukuze kwandiswe kakhulu umthamo futhi kuncishiswe isikhathi esisuka ekuqoqweni kwesampula kuya ekubikweni kwemiphumela.

Izixazululo ze-PCR ze-Ele Bam Bioscience zisetshenziswa emikhakheni ebanzi yokutholwa kwezifo ezithathelwanayo, zinikeza odokotela nabacwaningi amathuluzi athembekile okuhlonza amagciwane, amabhaktheriya, isikhunta, kanye namagciwane ayizimuncagazi. Ngesikhathi sobhubhane lwe-COVID-19, amakhithi e-rt PCR e-Ele Bam adlale indima ebalulekile emizamweni yokuhlola abantu abaningi, enikeza imiphumela enembile kubantu abanezimpawu nabangenawo, ngemikhawulo yokuthola evumela ukuhlonzwa kwezifo ekuqaleni kokuqubuka kwazo. Lobu buchwepheshe buye bashintshwa ukuze busetshenziselwe ukuqapha umkhuhlane wesizini, okwenza amalabhorethri ezempilo omphakathi akwazi ukulandelela izinhlobo zamagciwane ezisakazekayo futhi anikeze ulwazi ngezinqumo zokwakhiwa kwemithi yokugoma unyaka nonyaka. Ngaphandle kwamagciwane okuphefumula, izinkundla ze-Ele Bam zisetshenziselwa ukuhlola amagciwane atholakala egazini, okuhlanganisa i-hepatitis B virus, i-hepatitis C virus, kanye ne-human immunodeficiency virus, lapho ukutholwa okunembile kakhulu kubalulekile ukuvimbela ukudluliselwa kwesifo ngomdlalo wegazi nokufakelwa izitho. Inkampani inikeza ukuhlelwa kwamaphanele okwenziwe ngokwezidingo ze-epidemiological zesifunda, njengamaphanele agxile ezifweni ezishisayo ezifana ne-dengue, i-Zika, ne-chikungunya emalabhorethri asezindaweni ezinomkhuhlane. Ukuhlolwa ngakunye kwenziwa ukuhlolwa okunzulu kwe-analytical ukuze kuqinisekiswe ukuthi kuhlangabezana nezidingo zokusebenza ezibekwe izinhlangano zomhlaba wonke ezilawulayo, okuhlanganisa imikhawulo yokutholwa, ukuhambisana, ukunemba, nokuqina. Ngokuhlinzeka ngenkundla eyodwa engakwazi ukuphatha izinhlobo ezahlukahlukene ezinjalo, i-Ele Bam Bioscience isiza amalabhorethri ukuthi ahlanganise izinqubo zawo zokuhlola futhi anciphise ubunzima bokugcina izinhlelo eziningi zokuhlola ezihlukene.

Ngaphandle kokutholwa kwezifo ezithathelwanayo, amakhithi e-Ele Bam PCR asetshenziswa kabanzi ekuhlaziyeni uhlobo lofuzo nokuguquka kwezakhi zofuzo, enza abacwaningi nodokotela bakwazi ukuhlonza izinguquko zofuzo ezihambisana nokumelana nemithi, ukuthambekela ezifweni, kanye nokusabela ekwelashweni. Ukucacisa okuphezulu kwezindlela zama-enzyme ze-Ele Bam kuvumela ukuhlukaniswa okucacile phakathi kwe-single-nucleotide polymorphisms, okwenza amakhithi afanelekele izinhlelo zokusebenza ezifana nokuhlola izakhi zofuzo zokumelana nama-antibiotic kumagciwane ahlukanisiwe noma ukuhlonza izinguquko zofuzo ezibalulekile emitholampilo kumagciwane omdlavuza. Ezinhlelweni zokulinganisa ubuningi begciwane, izinhlelo ze-real-time PCR ze-Ele Bam zinikeza ukulinganisa okuphelele nokuhlobene ngomugqa omuhle kulo lonke uhla olubanzi lokusebenza, zisekela ucwaningo lokusebenzisana komphathi negciwane kanye nezinguquko zokuhlala kwamagciwane. Ikhono lokulinganisa ngokunembile umthwalo wegciwane ngokuhamba kwesikhathi linikeza abacwaningi ukuqonda ngempumelelo yemithi elwa namagciwane, ukuqhubeka kwezifo ezingapheli, kanye nomthelela wokungenelela komzimba. I-Ele Bam iphinde isekele izinhlelo zokusebenza ezikhethekile ezifana ne-digital PCR, lapho ukuhlukaniswa kwamasampula kube izinkulungwane zokusabela okungama-nanoliter kuvumela ukulinganisa okuphelele ngaphandle kwesidingo samajika ajwayelekile, okwandisa ukusetshenziswa kwezinto zazo. Emalabhorethri enza kokubili ucwaningo nokuhlola emitholampilo, ukuba nomthombo owodwa othembekile we-PCR reagents kwenza kube lula ukuthenga, kunciphisa ukuhluka phakathi kokuhlola, futhi kuhlela ukuqeqeshwa nokuxazulula izinkinga. Ukuzivumelanisa kobuchwepheshe be-Ele Bam kulezi zimo ezahlukene zokusebenza kugcizelela ukusebenza nokwethembeka okulethwa yinkampani emakethe yokutholwa kwezifo ngamangqamuzana.

I-Ele Bam Bioscience inamathela ezindinganisweni zokukhiqiza eziqinile eziqinisekisa ukuthi yonke ibhetshi yama-reagents e-PCR iletha ukusebenza okungaguquki, kusukela kubhetshi lokuqala kuye kweyinkulungwane. Izindawo zokukhiqiza zenkampani zisebenza ngaphansi kwezinhlelo zokuphatha ikhwalithi eziqinisekisiwe ze-ISO 13485, zilandela Imikhuba Emihle Yokukhiqiza elawula konke kusukela ekuthengeni izinto ezingavuthiwe kuya ekupakishweni nasekubhalweni komkhiqizo wokugcina. Ibhetshi ngalinye le-master mix, i-enzyme, ne-buffer lidlula ukuhlolwa okuphelele kokulawula ikhwalithi okuhlanganisa izilinganiso zomsebenzi we-enzymatic, i-pH, ubunyumba, namazinga e-endotoxin, kanye nokuhlolwa kokusebenza kusetshenziswa ama-nucleic acid ajwayelekile ayizingqinamba. Ukungaguquki kwebhetshi kuya ebhetshini kuqinisekiswa ngeziqhathaniso ezenziwa eceleni ezihlola izinkomba ezibalulekile zokusebenza njengamanani we-threshold cycle, ukusebenza kahle kokukhulisa, namandla esignali emazingeni amaningi ahlosiwe. Le ndlela eqinile yokuqinisekisa ikhwalithi isho ukuthi amalabhorethri angashintsha phakathi kwebhetshi yokukhiqiza ngaphandle kokudinga ukuhlela kabusha izinqubo zawo noma ukuphinda izivivinyo zokuqinisekisa, okonga isikhathi nezinsiza eziyigugu. I-Ele Bam iphinde igcine idatha ebanzi yokuzinza yomkhiqizo ngamunye, enezifundo zokuguga okusheshayo ezibikezela isikhathi sokuphila komkhiqizo ngaphansi kwezimo ezahlukene zokugcina, iqinisekisa ukuthi ama-reagents asebenza ngokuthembekile phakathi nesikhathi sawo esibekiwe sokuphelelwa yisikhathi. Emalabhorethri angaphansi kokugadwa kwemithetho, i-Ele Bam ihlinzeka ngezitifiketi ezinemininingwane zokuhlaziya nemibhalo yekhwalithi esekela ukulandelelwa kokuhlola nokuhambisana namazinga e-CLIA, CAP, ne-ISO 15189.

Ukuqinisekiswa kokusebenza okuzimele kuqinisekisa ngokuqinile ukwethembeka kwezixazululo ze-PCR ze-Ele Bam, ngezifundo ezenziwa emalabhorethri ezinkomba zangaphandle kanye nezikhungo zocwaningo zemfundo ephakeme emhlabeni wonke. Lokhu kuhlola ngokuvamile kuqhathanisa amakhithi e-Ele Bam nezindlela ezimisiwe zegolide usebenzisa amaphaneli amasampula omtholampilo ahlukaniswe kahle, okuhlanganisa izinhlobo ezimbili eziqondile nezingezona ezimelela izinhlobonhlobo zokugxila kwamagciwane kanye nezinhlobo ze-matrix. Idatha eshicilelwe evela kulezi zifundo ikhombisa ukuthi ukuhlolwa kwe-Ele Bam kuthola amazinga okuzwela nokuqina adlula u-95% ezinhlosweni ezinkulu ezifana ne-SARS-CoV-2, amagciwane omkhuhlane, kanye negciwane lesifo sokusha kwesibindi, kanti ama-coefficients okuhluka phakathi kokuhlola nangaphakathi kokuhlola ahlala engaphansi kuka-5%. Inkampani ibamba iqhaza ezinhlelweni zokuhlola ikhwalithi yangaphandle, njengalezo ezihlelwa yi-College of American Pathologists kanye ne-World Health Organization, ukuze iqhathanise imikhiqizo yayo namazinga omhlaba futhi ihlonze amathuba okuthuthukisa. Impendulo evela kulezi zinhlelo ifakwa ngokuhlelekile emgqeni wokuthuthukiswa komkhiqizo, iqhube ukuthuthukiswa okuqhubekayo kwezindlela ze-enzyme, ukwakheka kwe-buffer, kanye nemiklamo yokuhlola. I-Ele Bam iphinde yenze idatha yokusebenza itholakale kumakhasimende ngamaphepha obuchwepheshe kanye namanothi okusebenza, inikeze ukucaca mayelana namandla kanye nemikhawulo yomkhiqizo ngamunye. Ngokuhlanganisa ukulawulwa kwekhwalithi yangaphakathi okuqinile nokuqinisekiswa kwangaphandle okunomgomo, i-Ele Bam Bioscience inikeza amalabhorethri ukuzethemba okudingayo ukuze ancike emiphumeleni yawo ye-PCR ezinqumweni ezibalulekile zokuxilonga nocwaningo.

I-Ele Bam Bioscience inikeza ikhathalogi yemikhiqizo ephelele ehlanganisa ama-reagents e-PCR ngamanye, amafomethi amakhithi aphelele, kanye namaphakheji ahlanganisiwe wezinto zokusebenza nama-reagents aklanyelwe ukuhlangabezana nezidingo zamalabhorethri anoma yiliphi izinga. Amakhasimende angabuka uhla oluphelele lwemikhiqizo nge-Imikhiqizo ikhasi, lapho bazothola khona imininingwane eningiliziwe, ulwazi lokuhlela, kanye nezicelo ezinconyiwe zento ngayinye. Emalabhorethri anezidingo ezikhethekile, i-Ele Bam inikeza izinketho zokwenza ngokwezifiso ezivumela ukulungiswa kwezilandelaniso zama-primer nama-probe, ukukhethwa kombala, usayizi wokupakisha, kanye nokuhlanganiswa ngokwezifiso kwezihlanganisi eziningi zama-master mix zibe yizixazululo ezingomumo owodwa. Lokhu kuguquguquka kuqinisekisa ukuthi ngisho nemiklamo emisha kakhulu yokuhlola ingasekelwa ngaphandle kokuthi amalabhorethri adelele ukusebenza kahle noma ukusebenziseka kalula. Ukuze uqale ngomyalo wangokwezifiso, amakhasimende angaxhumana nethimba lezokuthengisa lobuchwepheshe le-Ele Bam nge-Uhloboikhasi lokuxhumana, lapho bengathumela khona izidingo zabo futhi bathole isilinganiso esenziwe ngokwezidingo zabo phakathi nezinsuku ezimbili zokusebenza. Inkampani iphinde inikeze amakhithi okuhlola avumela amakhasimende angaba khona ukuthi ahlole ama-reagent e-Ele Bam ezinkundleni zawo kanye nezinhlobo zamasampula zawo ngaphambi kokuzibophezela ekuthengeni okukhulu, okunciphisa ubungozi obuhambisana nokushintsha abathengisi. Izaphulelo zobuningi kanye nezivumelwano zama-oda angapheli ziyatholakala emalabhorethri asebenza kakhulu kanye namakhasimende ezikhungo, okunikeza ukubikezela kwezindleko kanye nokuphepha kochungechunge lokuhlinzeka ezinhlelweni zokuhlola eziqhubekayo.

Ukusekelwa kwezobuchwepheshe kuyisisekelo samava ekhasimende le-Ele Bam Bioscience, inethimba lososayensi abanama-PhD kanye nochwepheshe abanolwazi emkhakheni abatholakalayo ukusiza ekulungiseni ukuhlaziya, ukuxazulula izinkinga, nokuhlanganisa ukusebenza. Amakhasimende angathola izinsiza eziningi eziku-inthanethi ngokusebenzisa inkampani IkhayaIkhasi, okuhlanganisa imihlahlandlela yezinqubo, izifundo zevidiyo, amanothi wokusebenzisa, kanye nezizindalwazi zemibuzo evame ukubuzwa ezihlanganisa izinkinga ezivamile ezifana nokuklanywa kwama-primer, amapharamitha wokushintsha ukushisa, nokuhlaziywa kwedatha. Ezinseleleni eziyinkimbinkimbi, i-Ele Bam inikeza izikhathi zokubonisana okungokoqobo lapho uchwepheshe wezobuchwepheshe engabuyekeza idatha yokuhlola, aphakamise izinguquko zohlelo, futhi asize ekuklanyeni izifundo zokuqinisekisa ezenzelwe izidingo ezithile zekhasimende. Inkampani iphinde ibambe ama-webinars avamile kanye nama-workshops ahlanganisa izihloko ezinjengezindlela ezingcono zokuthuthukisa ukuhlolwa kwe-rt PCR, amasu okuthuthukisa ukuhlanganisa okuningi, kanye nezitayela ezisafufusa ekuxilongeni kwamangqamuzana. Amakhasimende abhalisela i-newsletter ye-Ele Bam athola izibuyekezo mayelana nokwethulwa kwemikhiqizo emisha, izibuyekezo zesofthiwe, kanye neminikelo yokukhangisa, okuqinisekisa ukuthi ahlala enolwazi ngezinto ezintsha zakamuva. Ukuze uthole ulwazi olwengeziwe mayelana nokuzibophezela kwenkampani ezingeni eliphezulu nokwaneliseka kwamakhasimende, vakashela i-Mayelana Nathi ikhasi, elinikeza umbono mayelana nomsebenzi we-Ele Bam, ithimba, kanye nobambiswano. Inhlanganisela yohlu olubanzi lwemikhiqizo, amandla okwenza ngokwezifiso, kanye nokusekelwa kobuchwepheshe okuzinikele kwenza i-Ele Bam Bioscience ibe nguzakwethu othembekile emalabhorethri emhlabeni wonke afuna izixazululo ze-PCR ezinokuzwela okuphezulu ukuze kutholakale ukuxilongwa okuthembekile.

Ama-Ele Bam PCR kits athola ukuzwela okuphezulu ngokuhlanganisa ukubopha kwe-primer okunamandla okuphezulu, i-thermostable polymerase enobunikazi ene-processivity ethuthukisiwe, kanye ne-optimized buffer chemistry enciphisa umsindo wesiginali. Lokhu kuvumela ukutholwa okungaguquki kwama-target copies angu-5 kuya kwangu-10 ngokuphendula ngakunye, okudlula amanye ama-kits avamile ezimweni zokuthola i-low-titer. Ukwakheka kwe-enzyme futhi kukhombisa ukumelana okukhethekile nezithiyo ezivamile ze-PCR, okuthuthukisa ukuzwela ezintweni eziyinselele ezinjengegazi, isikhwehlela, kanye ne-soil extracts.

Yebo, amasistimu e-PCR yesikhathi sangempela akwa-Ele Bam aklanyelwe futhi aqinisekisiwe ukusetshenziswa kocwaningo kanye nokuxilonga kwemitholampilo. Le mishini ihlangabezana namazinga afanele okulawula ukuze isetshenziswe emitholampilo, futhi inkampani ihlinzeka ngemibhalo ebanzi ehlanganisa izitifiketi zokuhlaziya kanye nemibiko yokuqinisekisa ukuze kusekelwe ukuhambisa imibhalo yokulawula. Amakhasimende asebenzisa izinkundla zakwa-Ele Bam ekuxilongeni kwemitholampilo ahlomula ngokuzwela okuphezulu nokukhiqiza kabusha okufanayo okwenza lezi zinhlelo zithandwe ezindaweni zocwaningo.

I-Ele Bam Bioscience isekela izinhlobo ezahlukene ze-PCR, okuhlanganisa i-conventional end-point PCR, i-real-time quantitative PCR (qPCR), i-one-step ne-two-step rt PCR, i-multiplex PCR, kanye nezinhlelo zokusebenza ze-digital PCR. Umkhiqizo ngamunye wenziwe ukuze unikeze ukusebenza okuphelele ngendlela ohloswe ngayo, kunezihlanganisi eziyizingqungqulu ezikhethekile ezitholakalayo kuma-template e-GC-rich, ukuhlolwa okuqondile kwamasampula, kanye nezinqubo zomsebenzi eziphezulu. Idizayini evulekile yenkampani iphinde ivumele ukuhambisana nezinto eziningi zangaphandle kanye nezinto ezisetshenziswayo.

Ukuhambisana kwe-lot-to-lot kuqinisekiswa ngokuhlolwa okuqinile kwekhwalithi okukala umsebenzi we-enzymatic, i-pH, ubunyumba, amazinga e-endotoxin, nokusebenza okusebenzayo ngokuqhathaniswa nama-nucleic acid ajwayelekile. I-loti ngayinye entsha iqhathaniswa eceleni neloti yangaphambilini kusetshenziswa amanani we-threshold cycle, ukusebenza kahle kokukhulisa, nezilinganiso zamandla esignali. Izinqubo zokukhiqiza eziqinisekisiwe ze-ISO 13485 ze-Ele Bam ziqinisekisa ukuthi izimo zokukhiqiza zilawulwa ngokuqinile kuwo wonke amabhachi.

Izinqubo zokuhamba ngokushesha ze-Ele Bam zivumela amapuleti ajwayelekile angama-96 noma angama-384 ukuthi aqedwe ngaphansi kwemizuzu engama-40, kuhlanganise nokuhlaziywa kwedatha. Lokhu kuphenduka okusheshayo kunikwa amandla yimojuli ethuthukisiwe yokushisa kwe-thermal enezinga lokukhuphuka okusheshayo nokudluliswa kokushisa okuphumelelayo, kuhlangene ne-polymerase enezikhathi zokunweba ezisheshayo. Kuma-amplicon amafushane noma izivivinyo ezithuthukisiwe, izikhathi zokusebenza zingancishiswa zibe ngemizuzu engama-25 kuya kwengama-30 ngaphandle kokulahlekelwa ubucayi noma ukucacisa.

Yebo, i-Ele Bam inikeza izinsiza eziphelele zokuklama ukuhlola ngokwezifiso ngethimba layo lokusekela ubuchwepheshe, okuhlanganisa ukuklama ama-primer nama-probe, ukulungisa i-multiplex panel, nokuqinisekisa nge-wet-lab. Amakhasimende angathumela ukulandelana kwe-target futhi athole umklamo wokuhlola oqinisekisiwe ngokugcwele kanye nokwakheka kwe-kit okwenziwe ngokwezifiso okuhambisana nezidingo zawo. Amathuluzi okuklama enkampani ahlola ukusabela okuphambene nezizinda zedatha ezinwetshiwe ukuze kuqinisekiswe ukucacisa okuphezulu.

Ama-Ele Bam PCR kits aklanyelwe ngefomethi evulekile ye-platform eqinisekisa ukuhambisana namathuluzi amaningi amakhulu e-real-time PCR avela kubakhiqizi abanjenge-Applied Biosystems, Bio-Rad, kanye ne-Roche. I-chemistry ye-buffer elungiselelwe kanye nokwakheka kwe-enzyme kusebenza ngokungaguquki kuma-thermal cyclers ahlukene kanye nezinhlelo zokuthola izibonakaliso. Abasebenzisi kufanele baqinisekise ukuhambisana nemodeli yethuluzi labo elithile besebenzisa imininingwane yobuchwepheshe enikezwe ekhasini lomkhiqizo.

Izikhungo zokukhiqiza ze-Ele Bam Bioscience zigunyazwe ngaphansi kwe-ISO 13485, okuyindinganiso yamazwe ngamazwe yezinhlelo zokuphatha ikhwalithi ekukhiqizweni kwemishini yezokwelapha. Inkampani futhi ilandela Izindlela Zokukhiqiza Ezinhle futhi ibamba iqhaza ezinhlelweni zangaphandle zokuhlola ikhwalithi ezihlelwe yizinhlangano ezifana ne-College of American Pathologists kanye ne-World Health Organization. Lezi zitifiketi nokubamba iqhaza kubonisa ukuzibophezela kwenkampani ekukhiqizeni ama-PCR reagents athembekile, anekhwalithi ephezulu.

Iningi lezinto zokusebenza ze-Ele Bam PCR, okuhlanganisa izingxube eziyinhloko nezixazululo zama-enzyme, kufanele zigcinwe ku--20°C esiqandisini esinezinga lokushisa elingaguquki, zivikelwe ekukhanyeni nasekushintsheni okuphindaphindiwe kokuncibilika nokuqina. Inkampani inikeza imiyalelo enemininingwane yokugcina kumthetho ngomkhiqizo ngamunye nasemqulwini wezobuchwepheshe ohambisana nawo. Ngaphansi kwezimo zokugcina ezifanele, izinto zokusebenza ze-Ele Bam zigcina ukusebenza ngokugcwele ngesikhathi sokuphila kwazo okubhalwe emthethweni, ngokuvamile izinyanga eziyi-12 kuye kwezingama-24 kusukela ngosuku lokukhiqizwa.

Amakhasimende angafinyelela ku-Ele Bam Bioscience nge-Uhlobo ikhasi lokuxhumana, elinikeza ifomu lokuhambisa izicelo zokusekelwa kobuchwepheshe, imibuzo yoku-oda, kanye nezidingo zezilinganiso ezenziwe ngokwezifiso. Inkampani iphinde igcine umtapo wolwazi ophelele ku-Ikhaya ikhasi, okuhlanganisa imihlahlandlela yemigomo, amanothi okusebenzisa, nezifundo zevidiyo. Ezinkingeni zobuchwepheshe eziphuthumayo, ithimba lokusekela livame ukuphendula ngaphakathi kosuku olulodwa lwebhizinisi.