I-Polymerase chain reaction, eyaziwa kakhulu nge-PCR, ingesinye sezindlela eziguqule kakhulu i-molecular biology yesimanje, inika amandla okukhulisa ngokushesha ukulandelana okuthile kwe-DNA kusukela ezintweni ezincane kakhulu zokuqala. Kusukela yasungulwa, i-PCR iguqule imikhakha esukela ekuxilongweni kwemitholampilo kuya kwezobuchwepheshe bezobugebengu, futhi ukubaluleka kwayo kuya kukhula njengoba kuvela izinhlobo ezintsha kanye nokusetshenziswa okusha. I-Ele Bam Bioscience, umkhiqizo ozinikele ngaphansi kwesambulela se-YazhuBIO, izibeke phambili kule teknoloji ngokuthuthukisa amakhithi e-PCR ahlinzeka ngokuzwela okukhethekile, isivinini, nokwethembeka emalabhorethri emhlabeni wonke. Ukuzinikela kwenkampani ekuthuthukiseni ubuchwepheshe be-PCR kubonakala kuwo wonke umkhiqizo abawukhiphayo, kusukela kuma-master mixes kuya ezinhlelweni eziphelele zamakhithi ezenzelwe imisebenzi ethile. Ngokuhlanganisa ucwaningo olusezingeni eliphezulu namazinga aqinile okukhiqiza, i-Ele Bam Bioscience iqinisekisa ukuthi abacwaningi nodokotela bangathemba imiphumela yabo, noma ngabe basebenza ocwaningweni oluyisisekelo noma ekuxilongweni okubucayi ngesikhathi. Lokhu kuzinikela kwikhwalithi nokusungula izinto ezintsha kwenza i-Ele Bam Bioscience ibe umlingani obalulekile kunoma yiliphi ilabhorethri elifuna ukuthuthukisa amandla alo e-PCR.

Ukuqonda izidingo ezahlukene zomphakathi wesayensi, i-Ele Bam Bioscience ilungiselele imikhiqizo yayo ukuze ibhekane nezinselele ezivame kakhulu ekukhuliseni kwe-PCR, njengokubekezelela izithiyo, izakhiwo zesikhombi esiyisisekelo, kanye nokukhuliswa okungaqondile. Lolu hlelo lusebenzisa ulwazi olunzulu kanye nokufinyelela emhlabeni wonke kwe-YazhuBIO, igama elithembekile ezintweni zokusebenza zaselabhorethri kanye nezinto zokuphila ze-biotechnology, ukuze kusatshalaliswe imikhiqizo yalo ngempumelelo futhi kunikezwe usizo oluphelele lwezobuchwepheshe. Abacwaningi abakhetha amakhithi e-PCR e-Ele Bam Bioscience bathola ukufinyelela hhayi kuphela kumareagent asebenza kahle kakhulu kodwa nasesizindalwazi esikhulu solwazi olubasiza ukuthi balungise izinkinga futhi bathuthukise izinqubo zabo. Ukugxila kwenkampani ekufundiseni amakhasimende kanye nokuxazulula izinkinga ngokubambisana kuqinisekisa ukuthi wonke umsebenzisi angathola imiphumela emihle, kungakhathaliseki izinga labo lolwazi nge-PCR. Le ndlela ephelele yokuthuthukisa umkhiqizo kanye nensizakalo yamakhasimende ithole i-Ele Bam Bioscience idumela lokugqama emkhakheni wokuncintisana wamareagent e-molecular biology.

Inhliziyo yalo lonke uhlelo oluyimpumelelo lwe-PCR iyi-enzyme ye-DNA polymerase, futhi i-Ele Bam Bioscience itshale imali eningi ekwakheni ama-polymerase ane-high-fidelity ahlinzeka ngokunemba nokusebenza okungenakuqhathaniswa. Amafomula abo obunikazi be-high-fidelity DNA polymerase abonisa umsebenzi oqinile wokuhlola amaphutha, anciphisa kakhulu izinga lamaphutha ngesikhathi sokukhulisa futhi aqinisekise ukuthi izinhlelo zokusebenza ezingezansi ezifana nokuhlanganisa, ukulandelana, nokuhlaziywa kokuvezwa kofuzo zinikeza idatha enokwethenjelwa. Ngaphezu kwe-polymerase uqobo, i-Ele Bam Bioscience ihlinzeka ngezinhlelo ze-buffer ezilungiselelwe ezinokulinganisela ngokucophelela ukuze kugcinwe i-pH efanele, ukugxila kukasawoti, nokutholakala kwe-cofactor ukuze kutholakale umsebenzi omkhulu we-enzyme kanye nokucaciswa. Lawa ma-buffer aklanyelwe ukusebenza ngokuhambisana nezinhlobo eziningi zethempulethi, okuhlanganisa izifunda ezinothe kakhulu nge-GC kanye ne-genomic DNA eyinkimbinkimbi, okuyizinto ngokwesiko ezinzima ukuzikhulisa. Ngaphezu kwalokho, inkampani ifaka izithasiselo ezithuthukisiwe kanye nezithuthukisi kuma-master mixes ayo ukuze inqobe izakhiwo ezesibili futhi ithuthukise isivuno, okwenza amakhithi ayo afanele ngisho nezinhloso eziyinselele kakhulu ze-PCR. Ngokulawula yonke ingxenye yengxube yokusabela, i-Ele Bam Bioscience iqinisekisa ukusebenza okungaguquki kuma-thermal cyclers ahlukene kanye nezimo zaselabhorethri.

Esinye sezici ezivelele zohlu lwezingxenye ze-Ele Bam Bioscience yizinhlobo zazo ze-Taq polymerase ezenziwe ngokukhethekile, ezenzelwe izinhlelo zokusebenza ezithile ezifana ne-hot-start PCR, ukukhulisa amandla amade, kanye ne-multiplex PCR. I-hot-start Taq polymerase iguqulwa ngamakhemikhali noma iboshelwe amasosha omzimba ukuze ingasebenzi emazingeni okushisa asekamelweni, ivimbele ukuqala okungaqondile nokwakheka kwe-primer-dimer ngesikhathi sokuhlela ukusabela nokushisa kokuqala. Lokhu kusungula kuthuthukisa kakhulu ukucacisa nokuzwela, ikakhulukazi ekuphenduleni okuningi lapho izintambo eziningi ze-primer zikhona ngasikhathi sinye. Izinhlelo zokugcina ezilungiselelwe ezinikezwa ngalawa ma-enzyme zihlanganisa ukugxila kwe-MgCl₂ okulungiswe kahle ukuze kulinganise umsebenzi nokwethembeka, kanye nezinto ezinzile ezivikela i-enzyme ngesikhathi semijikelezo yokushisa nokuncibilikisa kanye nokugcinwa isikhathi eside. I-Ele Bam Bioscience iphinde inikeze amaphakethe e-buffer ahlukene avumela abacwaningi ukuthi benze ngokwezifiso izimo zabo zokusabela ezinhlelweni zokusebenza ezikhethekile, okubanikeza ukuguquguquka ukuthi balungise izinqubo zabo. Lokhu kunaka imininingwane ekwakhiweni kwezingxenye kubonisa ukuqonda okujulile kwenkampani mayelana nezinto eziyinkimbinkimbi ze-biochemical eziqhuba ukukhuliswa okuyimpumelelo kwe-PCR.

I-Ele Bam Bioscience ithuthukise umkhiqizo ophelele ohlanganisa izinsika ezintathu ezinkulu zobuchwepheshe be-PCR: i-PCR evamile yokuhlaziya ukuphela, i-quantitative PCR (qPCR) yokuqapha isikhathi sangempela, kanye ne-reverse-transcription PCR (RT-PCR) yokuhlaziya i-RNA. Amakhithi abo avamile e-PCR aklanyelwe imisebenzi evamile yokwandisa, enikeza ukusebenza okuqinile ngokudinga ukulungiswa okuncane, futhi atholakala ngezindlela ezahlukene ezihlanganisa i-2X master mixes kanye namasethi ezingxenye ngazinye. Kubacwaningi abadinga ukulinganisa ukuvezwa kofuzo noma ukuthola amagciwane ngokunemba okuphezulu, amakhithi e-qPCR ka-Ele Bam ahlanganisa amakhemikhali athuthukile e-fluorescent afana ne-SYBR Green kanye nokutholwa okususelwa kuprobe, kanye nama-buffers athuthukisiwe akhulisa isignali ye-fluorescent futhi anciphise umsindo wangemuva. Amakhithi e-RT-PCR avela ku-Ele Bam Bioscience ahlanganisa ama-enzyme e-reverse transcriptase asebenza kahle nezingxenye zawo eziqinisekisiwe ze-PCR, enika amandla ukuguqulwa okungenazihibe kwe-RNA kuye ku-cDNA okulandelwa ukwandiswa ngefomethi ye-single-tube. Lo msebenzi owenziwe lula wehlisa izinyathelo zokuphatha kanye nengozi yokungcola, okwenza kufaneleke kakhulu ekuxilongweni kwemitholampilo kanye nezinhlelo zokuhlola eziphezulu. Ikhithi ngayinye emgqeni womkhiqizo ihambisana nezinqubo ezinemininingwane kanye namanothi okusebenza aqondisa abasebenzisi ekumiseni, imingcele ye-thermal cycling, kanye nokuhlaziywa kwedatha.

Ukuhlukahluka komugqa womkhiqizo we-PCR we-Ele Bam nakho kubhekana nesidingo esikhulayo sezinhlelo zokusebenza ezikhethekile ezifana ne-digital PCR, i-fast cycling PCR, kanye ne-direct PCR evela kumasampula angahluziwe. Amakhithi abo okugijima asheshayo asebenzisa ama-polymerase enziwe ngobunjiniyela kanye nama-buffers akhethelwe ngokukhethekile anciphisa kakhulu izikhathi zokwelula ngaphandle kokulahlekelwa isivuno noma ukucaciswa, okuvumela ukukhuliswa okuphelele ngaphansi kwemizuzu engama-30 ezinhlosweni eziningi. Emalabhorethri enza i-high-throughput genotyping noma ukuhlolwa kwamagciwane, i-Ele Bam inikeza amakhithi e-multiplex PCR ahlanganiswe ngaphambilini ukuze akhuliswe kanyekanye izinhloso eziyishumi ekuphenduleni okukodwa. Inkampani futhi ihlinzeka ngamakhithi e-reverse-transcription quantitative PCR (RT-qPCR) ahlanganisa ukuzwela kwe-qPCR nokuguquguquka kwe-RT-PCR, okuvumela ukulinganiswa okunembile kwezinhloso ze-RNA kusuka kumavolumu amancane esampula. Ngokuhlukanisa imikhiqizo yabo ngokuya ngezinhlobo ze-PCR ezivame ukusetshenziswa ocwaningweni nasekuhloleni, i-Ele Bam Bioscience yenza kube lula kubathengi ukukhetha ikhithi efanele izidingo zabo ezithile zokuhlola. Lokhu kuhlukaniswa komkhiqizo okucatshangelwe, kuhlangene nekhwalithi engaguquki kuwo wonke umugqa, kwenze i-Ele Bam yaba ngumthombo oya kuyo wama-reagents e-PCR emalabhorethri emhlabeni wonke.

Enye yezizathu eziheha kakhulu zokukhetha ama-PCR kits akwa-Ele Bam Bioscience ukuzwela kwawo okukhethekile, okuvumela abacwaningi ukuthi bathole amakhophi ayisithupha kuya kwayishumi we-DNA ekhethiwe emlandweni oyinkimbinkimbi, ikhono elibalulekile ezinhlelweni ezinjengokutholwa kwezifo kusenesikhathi nokuhlaziywa kwezinguquko ezingavamile. Lokhu kuzwela okukhulu kutholakala ngenhlanganisela yama-polymerase asebenza kahle kakhulu, i-buffer chemistry elungiselelwe, kanye nokulawulwa kwekhwalithi okuqinile okunciphisa ubukhona bezinto ezingcolisayo eziyizingqinamba kuma-reagents. Ukuzwela okuthuthukisiwe kwama-kits akwa-Ele Bam kuhumushela ngqo emiphumeleni ethembekile, kunciphisa inani lemiphumela engamanga futhi kwandisa ukuzethemba ekuchazweni kwedatha eyenzelwe phansi. Enye inzuzo enkulu ingcuphe encishisiwe yokungcoliswa, okubhekwa yi-Ele Bam ngokusebenzisa i-dUTP kanye nezinhlelo ze-uracil-DNA glycosylase (UDG) kuma-kits ayo amaningi ukuvimbela ukungcoliswa okuvela ekuphindaphindweni kwangaphambilini. Ukwengeza, ama-master mixes enkampani enziwe ukunciphisa ukukhiqizwa kwe-aerosol ngesikhathi sokupipetha, futhi ahlinzeka ngemihlahlandlela ecacile yokuhlukaniswa komsebenzi ukuze kusizwe amalabhorethri agcine izindawo ezihlanzekile ze-PCR.

Izikhathi zokujikeleza okusheshayo zimele insika yesithathu yenzuzo yokuncintisana ye-Ele Bam, lapho amakhithi abo asheshayo e-PCR enza ukuthi ukukhuliswa okuphelele kuthathe imizuzu engu-15 kuya kwengu-30 kuphela ezinhlosweni eziningi ezijwayelekile, okwandisa kakhulu umthamo waselabhorethri futhi kunciphisa isikhathi sokuthola imiphumela yokuhlolwa okubalulekile kokuxilonga. Lezi zinqubo zokujikeleza okusheshayo zenzeka ngenxa yama-polymerase enziwe ngobunjiniyela abanomkhawulo wokukhula ophakeme kanye nama-buffers agcina ukuzinza kwe-enzyme emazingeni okushisa aphakeme, okuvumela izinyathelo ezimfushane zokunciphisa nokuhlanganisa. Inhlanganisela yokuzwela okuthuthukisiwe, ukulawulwa okuqinile kokungcoliswa, nokujikeleza okusheshayo kwenza amakhithi e-PCR e-Ele Bam Bioscience afaneleke kakhulu emalabhorethri emitholampilo nasezikhungweni zocwaningo ezigcina izinamba ezinkulu zamasampula ngaphansi kwezikhathi eziqinile. Ngaphezu kwalokho, ukuzinikela kwenkampani ekuhambisaneni kwe-lot-to-lot kuqinisekisa ukuthi uma inqubo isiqinisekisiwe, ingaphindwa ngokuthembekile emabhendini ahlukene amakhithi, okonga isikhathi ekulungiseni kabusha nasekulawuleni ikhwalithi. Lezi zinzuzo ezibalulekile, ezisekelwa idatha eningi yokuqinisekisa kanye nobufakazi bamakhasimende, zibeka i-Ele Bam Bioscience njengomholi emakethe yama-reagents e-PCR.

Ama-PCR kits e-Ele Bam Bioscience asetshenziswa emikhakheni ehlukahlukene, lapho ukutholwa kwezifo ezibangelwa amagciwane kungenye yezindawo ezibaluleke kakhulu lapho ukuzwela okuphezulu kanye nesivinini kwenza umehluko obonakalayo emiphumeleni yeziguli kanye nezimpendulo zezempilo zomphakathi. Amalabhorethri emitholampilo asebenzisa ama-RT-PCR kits e-Ele Bam ukuze athole amagciwane afana nomkhuhlane, i-HIV, kanye nezifo ezintsha eziqubuka, ezizuzela emandleni amakits okukhulisa ama-RNA target ngokwethembeka okuphezulu nangokunciphisa ukuvinjelwa okuvela kumasampula emitholampilo. Amandla e-multiplex PCR ama-Ele Bam enza ukuthi kutholakale amagciwane amaningi ngesikhathi esisodwa, okonga isikhathi nezinto zokusebenza kuyilapho kunikeza ulwazi oluphelele lokuxilonga. Ngaphandle kokuhlola izifo ezithathelwanayo, imikhiqizo ye-PCR ye-Ele Bam isetshenziswa kakhulu ekuhlaziyweni kwe-gene expression, lapho abacwaningi bekhala ama-mRNA transcripts ukuze baqonde izinqubo zamaseli, izindlela zezifo, kanye nezimpendulo zemithi. Ama-quantitative PCR kits avela ku-Ele Bam ahlinzeka ngemiphumela enembile nephindaphindekayo ebangeni elibanzi, okuvumela ukukala okunembile kwama-transcripts akhulunywa kakhulu kanye nalawo anobuningi obuphansi.

Ngaphandle kwalezi zinhlelo eziyinhloko, amakhithi e-PCR e-Ele Bam Bioscience asekela izinhlobonhlobo zokusetshenziswa okukhethekile okuhlanganisa ukuhlola uhlobo lofuzo ukuze kuhlolwe izifo zofuzo, ukuhlaziywa kwe-DNA yobufakazi obusemthethweni, ukuhlola ukuphepha kokudla ukuze kutholwe amagciwane angcolisayo, kanye nokuqapha imvelo kwamanzi nesampula zomhlabathi. Ukuqina kwamakhithi kuwenza afaneleke ukukhuliswa okuqondile kusuka kuma-crude lysates kanye nezampula eziyinkimbinkimbi zemvelo, kunciphisa isidingo sezinyathelo ezinzulu zokuhlanzwa kwe-DNA. Abacwaningi futhi bathembela kumakhithi e-PCR e-high-fidelity e-Ele Bam emisebenzini yokuklona kanye nokuguqula izakhi zofuzo, lapho ukukhuliswa okunembile kubalulekile ukuze kugcinwe ubuqotho bokulandelana kwezakhi. Imikhiqizo yenkampani ibalulekile ngokulinganayo ekuxilongweni kwezilwane, ku-biotechnology yezolimo, kanye nocwaningo lwezemfundo, okubonisa ukusebenza okuhlukahlukene kobuchwepheshe bayo be-PCR emikhakheni ehlukene. Ngokuhlinzeka ngezindlela ezithile zokusebenza kanye nokuhola kobuchwepheshe, i-Ele Bam Bioscience iqinisekisa ukuthi abasebenzisi bangakwazi ukujwayeza amakhithi abo ezindleleni ezintsha ngokuzethemba. Lokhu kusebenza okubanzi, kuhlangene nokuzinikela kwenkampani ekusunguleni izinto ezintsha, kusho ukuthi njengoba izindlela ezintsha ezisekelwe ku-PCR zivela, i-Ele Bam ikulungele ukuzisekela ngama-reagents akhethekile kanye nolwazi lobungcweti.

I-Ele Bam Bioscience inohlelo olubanzi lokulawula ikhwalithi oluhlola yonke i-lot yamakhithi e-PCR ngobumsulwa, umsebenzi, ukuqondisa, kanye nokuzwela ngaphambi kokukhishwa, iqinisekisa ukuthi amakhasimende athola imikhiqizo esebenza ngokungaguquki ngokwemigomo. I-batch ngayinye ihlolwa ngokusebenza okuqinile ngezithenjwa ezijwayelekile, kanye nezithenjwa eziyinselele ezihlanganisa ukulandelana okunothe kwe-GC kanye nezinhloso eziphansi zekhophi, ukuqinisekisa ukuthi amakhithi ahlangabezana namazinga aphezulu okusebenza enkampani. Lokhu kuzibophezela ekhwalithini kunweba nasezintweni ezingavuthiwe ezisetshenziswa ekukhiqizeni, ezitholakala kubathengisi abahlonishwayo futhi zihlolwe lapho zifika ukuze kugwenywe ukuhluka emkhiqizweni wokugcina. Izindawo zokukhiqiza zenkampani zisebenza ngaphansi kwezinhlelo eziqinile zokuphatha ikhwalithi ye-ISO, futhi zonke izinqubo zokukhiqiza ziyabhalwa futhi zihlolwe njalo ukuze kugcinwe ukuthobela imithetho yamazwe ngamazwe. Amakhasimende angathola izitifiketi ezinemininingwane yokuhlaziya nge-lot ngayinye kuwebhusayithi ye-YazhuBIO, okuhlinzeka ngokungafihli okugcwele kanye nokulandeleka kwezinto zabo zokusebenza.

Ngaphandle kokuqinisekisa ikhwalithi, i-Ele Bam Bioscience inikezela ngezinsizakalo eziphelele zokusekela amakhasimende ezihlanganisa izintambo zosizo lobuchwepheshe, imihlahlandlela yokuxazulula izinkinga eku-inthanethi, kanye nezingxoxo zokwenza ngcono izinqubo eziqondene nawe. Ithimba lokusekela linabososayensi abanolwazi olunzulu nge-PCR kanye ne-molecular biology, abangasiza amakhasimende ukuthi ahlonze izinkinga ezinjengokukhuliswa okubuthakathaka, amabhendi angacacile, noma ukuvinjelwa okuvela kumasampula. I-Ele Bam iphinde inikeze ama-webinars okuqeqesha njalo kanye nezincwadi zokusebenza ezihlanganisa izindlela ezingcono kakhulu zokumisa i-PCR, ukwenza ngcono ukushintshashintsha kwezinga lokushisa, kanye nokuhlaziya idatha, okunika amandla abasebenzisi ukuthi bathole okuningi emakithini abo. Emalabhorethri adinga ukuqinisekisa izivivinyo ezintsha, inkampani ingahlinzeka ngamasampula okuhlola futhi isize ngokuklama nokwenza ngcono izivivinyo. Le nhlanganisela yokulawula ikhwalithi okuqinile kanye nokusekela amakhasimende okusabelayo iqinisekisa ukuthi ama-PCR kits e-Ele Bam Bioscience ahlinzeka ngemiphumela ethembekile kunoma yiliphi ilabhorethri, nokuthi usizo luhlala lukhona lapho ludingeka. Ukuze uthole ulwazi olwengeziwe mayelana nokuzibophezela kwenkampani ekhwalithini nasekusungulweni kwezinto ezintsha, vakashela i-Mayelana Nathi ikhasi kuwebhusayithi ye-YazhuBIO.

Ama-PCR kits e-Ele Bam Bioscience amelela ukuhlanganiswa okunamandla kobuchwepheshe obuthuthukisiwe be-enzyme, ikhemistri ye-buffer elungiselelwe kahle, nokuqinisekiswa kwekhwalithi okuqinile, okuletha ukuzwela okuphezulu, isivinini, nokwethembeka okudingwa amalabhorethri anamuhla. Umkhiqizo ophelele wenkampani uhlanganisa i-PCR ejwayelekile, i-quantitative PCR, ne-reverse-transcription PCR, kanye nezinhlobo ezikhethekile zokwenza i-multiplexing nokujikeleza okusheshayo, okwenza kube lula ukuthola isixazululo esifanele kunoma yisiphi isicelo. Ngokubeka phambili ukuzwela okuthuthukisiwe, ukunciphisa ubungozi bokungcoliswa, nezikhathi zokujikeleza ezisheshayo, i-Ele Bam ibhekana nezinselelo eziphuthumayo ekukhuliseni i-PCR futhi inika abacwaningi amandla okuthola imiphumela enembile ngempumelelo enkulu. Ama-kits asetshenziswa kabanzi ekutholeni izifo, ukuhlaziywa kokuvezwa kwezakhi zofuzo, ukuhlukaniswa kwezakhi zofuzo, neminye imikhakha eminingi, okubonisa ukusebenza kwawo okuhlukahlukene nokusebenza okuqinile ezinhlotsheni ezahlukene zamasampula nezimo zokuhlola. Ngokugxila kakhulu ekulawuleni ikhwalithi nasekusekeleni amakhasimende, i-Ele Bam Bioscience, esekelwa ingqalasizinda esunguliwe ye-YazhuBIO, ayinikezi nje izinto zokusebenza kodwa futhi nobuchwepheshe nezinsiza ezidingwa ososayensi ukuze baphumelele. Ukuze uhlole uhla oluphelele lwama-PCR kits e-Ele Bam Bioscience nemikhiqizo ehlobene, vakashela ku-Imikhiqizo ikhasi futhi uthole ukuthi lezi zixazululo ezintsha zingakuthuthukisa kanjani amandla elabhorethri yakho. Ukuze uthole izibuyekezo zakamuva nokuqonda okusetshenziswayo, hlola i-Izindaba section regularly, and learn more about the brand's mission and values on the Uhlobo ikhasi.

I-Ele Bam Bioscience inikeza amakhithi e-PCR ejwayelekile, i-PCR yobuningi (qPCR), i-PCR yokuguqula emuva (RT-PCR), i-PCR yezinhlobonhlobo, i-PCR yokugijima ngokushesha, ne-PCR yedijithali. Lolu hlu olubanzi luhlanganisa zonke izinhlobo ezinkulu ze-PCR ezisetshenziswa ocwaningweni nasekuhloleni, okukuvumela ukuthi ukhethe ikhithi efanele isicelo sakho esithile, noma ngabe ukhulisa i-DNA, ulinganisa i-RNA, noma uthola izinhloso eziningi ngesikhathi esisodwa.

Amakhithi e-Ele Bam PCR aklanyelwe ukuthola amakhophi amancane afika kweyodwa kuya kwayishumi we-DNA eyinhloso endaweni eyinkimbinkimbi, okwenza afaneleke kakhulu ekuhlaziyeni izinguquko ezingavamisile, ekutholeni amagciwane kusenesikhathi, nasezifundweni zokuvezwa kwezakhi zofuzo ezinama-transcripts amancane. Ukuzwela okuthuthukile kutholakala ngokusebenzisa ukwakheka kwe-polymerase okulungiselelwe, amasistimu e-buffer athuthukile, kanye nokulawulwa kwekhwalithi okuqinile okunciphisa ukungcola okuphazamisayo.

Yebo, i-Ele Bam Bioscience ihlinzeka ngamakhithi e-RT-PCR ne-RT-qPCR ahlanganisa ama-enzyme e-reverse transcriptase asebenza kahle nezingxenye ze-PCR ezifakazelwe ukuze kuguqulwe i-RNA ibe yi-cDNA futhi kwandiswe. Lawa makhithi enzelwe ukulinganisa okuthembekile kwama-RNA target kusuka kumasampula amancane futhi afanele ukuhlaziywa kwe-gene expression, ukutholwa kwe-viral RNA, nezinye izinhlelo zokusebenza ezisekelwe ku-RNA.

Yebo, amakhithi amaningi e-Ele Bam Bioscience PCR afaka izinhlobo ze-Taq polymerase ezinokwethembeka okuphezulu, kuhlanganise ne-hot-start formulations ezivimbela ukwandiswa okungaqondile ngesikhathi sokulungiselela ukusabela. Lawa ma-Taq polymerase enziwe ngobunjiniyela anikeza umsebenzi oqinile wokuhlela amaphutha, amazinga asheshayo okwandisa, nokubekezelela okuhle kakhulu kuma-inhibitors, okuqinisekisa ukwandiswa okunembile nokusebenza kahle kuzo zonke izinhlobo zamathempulethi.

Impela. I-Ele Bam Bioscience inikeza amakhithi e-multiplex PCR athuthukisiwe ahlanganiswe ngaphambilini ukuze akhulise izinhloso ezifinyelela kweziyishumi ngesikhathi esisodwa. Izinhlelo zamabhafa athuthukile nama-hot-start polymerases anciphisa ukwakheka kwe-primer-dimer nokukhuliswa okungaqondile, okuletha imiphumela ethembekile ye-multiplex yezinhlelo zokusebenza ezinjengokuhlolwa kwamaphathogeni nokuhlaziya uhlobo lofuzo.

I-Ele Bam ibhekana nokungcola ngezindlela eziningi, okuhlanganisa ukufaka i-dUTP kanye ne-uracil-DNA glycosylase (UDG) ezinhlelweni eziningi ukuze kuvinjelwe ukukhuliswa okudluliselayo, izingxube eziyinhloko ezenziwe ukunciphisa ukukhiqizwa kwe-aerosol, kanye nemihlahlandlela ecacile yokusebenza esiza amalabhorethri agcine izinqubo ezihlanzekile ze-PCR. Lezi zinyathelo zinciphisa kakhulu imiphumela engamanga futhi zithuthukisa ukwethembeka komphumela.

Amakhithi e-PCR asheshayo e-Ele Bam enza ukuthi ukukhuliswa okuphelele kuthathwe isikhathi esingangemizuzu engu-15 kuye kwengu-30 kuphela ezinhlosweni eziningi ezijwayelekile, kusetshenziswa ama-polymerase enziwe ngobunjiniyela anezinga eliphezulu lokwelula kanye nama-buffer afanele agcina ukuzinza kwe-enzyme emazingeni okushisa aphakeme. Lokhu kuphenduka okusheshayo kwandisa umthamo waselabhorethri futhi kubaluleke kakhulu ekuhloleni okubucayi ngesikhathi.

Yebo, amakhithi e-Ele Bam Bioscience enziwe ukumelana nezithiyo ezijwayelekile ze-PCR ezitholakala kuma-crude lysates, igazi, inhlabathi, nezicubu zezitshalo, kunciphisa isidingo sokuhlanzwa okujulile kwe-DNA. Izinhlelo zama-buffer eziqinile kanye nezithako ezengeziwe zisiza ukunqoba imiphumela evimbelayo, okwenza amakhithi afaneleke ukukhuliswa okuqondile kusuka kumasampula ayinkimbinkimbi emvelweni nawomtholampilo.

Yonke i-lot yamakithi e-Ele Bam PCR ihlolwa ngokuqinile ukusebenza kwayo ngokuhlanzeka, ukusebenza, ukuqondisa, nokuzwela kusetshenziswa izifanekiso ezijwayelekile neziyinselele. Izinto zokusetshenziswa zitholakala kubathengisi abathembekile futhi ziyahlolwa lapho zitholwa, futhi ukukhiqiza kulandela amazinga okuphatha ikhwalithi ye-ISO. Izitifiketi zokuhlaziya ziyatholakala ku-lot ngayinye ngewebhusayithi ye-YazhuBIO, okuqinisekisa ukulandeleka okugcwele.

I-Ele Bam Bioscience inikeza ukwesekwa kwamakhasimende okuphelele ngezintambo zosizo lobuchwepheshe, imihlahlandlela yokuxazulula izinkinga eku-inthanethi, izingxoxo zokwenza ngcono izinqubo ezenzelwe wena, nama-webinar avamile. Ithimba lokweseka linabantu abangososayensi bezinto eziphilayo abanolwazi oluningi abangasiza ekumiseni ukuhlola, ukwenza ngcono ukushisa okujikelezayo, nokuhlaziya idatha. Ungavakashela futhi ikhasi elithi Ikhaya ukuze uthole imininingwane yokuxhumana nezinsiza ezengeziwe.